Journal: Frontiers in Cardiovascular Medicine

Article Title: PACAP regulates VPAC1 expression, inflammatory processes and lipid homeostasis in M1- and M2-macrophages

doi: 10.3389/fcvm.2023.1264901

Figure Lengend Snippet: List of used antibodies.

Article Snippet: Rabbit anti-mouse CD163 , Orb182468 , Biorbyt, Cambridge, UK , 1:100.

Techniques:

Journal: Frontiers in Cardiovascular Medicine

Article Title: PACAP regulates VPAC1 expression, inflammatory processes and lipid homeostasis in M1- and M2-macrophages

doi: 10.3389/fcvm.2023.1264901

Figure Lengend Snippet: Effects of PACAP deficiency on the distribution and amount of different MΦ subtypes of atherosclerotic plaques in the aortic arch. Immunohistomorphometric analyses of atherosclerotic lesions in ApoE −/− and PACAP −/− /ApoE −/− mice after SC or CED. Analyses of VPAC1 ( A ), CD86 ( B ), CD68 ( C ), and CD163 ( D ) on cryosections of the aortic arch and representative photomicrographs ( E–T ) are shown. The graph bar is given as the mean. Black dots represent single measurement. ( n = 3–8). * p ≤ 0.05 vs. ApoE −/− after 30 weeks of SC; # p ≤ 0.05; ### p ≤ 0.001 vs. ApoE −/− after 20 weeks of CED. SC, standard diet; CED, cholesterol-enriched diet; n, number of experimental animals; Black arrows, positive reaction; asterisks, vessel lumen. Scale bar = 10 μm; Overview screen scale bar = 100 µm.

Article Snippet: Rabbit anti-mouse CD163 , Orb182468 , Biorbyt, Cambridge, UK , 1:100.

Techniques:

Journal: Frontiers in Physiology

Article Title: Branched-chain amino acids promotes the repair of exercise-induced muscle damage via enhancing macrophage polarization

doi: 10.3389/fphys.2022.1037090

Figure Lengend Snippet: BCAAs promote M1 and M2 polarization of macrophages during EIMD repair. (A–D) BCAAs promoted CD68/CD163 expression in macrophages during EIMD repair as revealed by immunohistochemical analysis (Scale Bar = 100 um). (E,F) The effects of BCAAs on serum levels of inflammatory cytokines, namely, IL-6, and IL-10 during EIMD repair. Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01 vs. PLA CON; & p < 0.05, && p < 0.01 vs. BCAA CON; # p < 0.05, ## p < 0.01 between PLA and BCAA.

Article Snippet: The sections were incubated with the following primary antibodies for 4 h at room temperature: rabbit anti-mouse CD68 (1/300 dilution; CST) and rabbit anti-mouse CD163 (1/500 dilution; CST).

Techniques: Expressing, Immunohistochemical staining